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Knockdown of HDAC6 attenuates Btz-induced autophagy initiation in HNSCC cells. A, Western blotting of acetyl α-tubulin (Lys-40), α-tubulin, p-mTOR (Ser-2448), mTOR, p-ULK1 (Ser-317), ULK1, p-Beclin1 (Ser-93/96), Beclin1, and GAPDH in SCC1 cells following Btz treatment. B, Western blotting of FLAG, acetyl α-tubulin (Lys-40), tubulin, p-mTOR (Ser-2448), mTOR, LC3, and GAPDH in HDAC6KD SCC1 cells following Btz treatment.
