FIGURE 4.

Peptide stapling can increase affinity of RalB binding. ITC data for stapled peptides titrated into RalB·GMPPNP at 298 K: SP1 (A), SP2 (B), SP3 (C), SP4 (D), SP5 (E), and SP6 (F). The parameters for the fit for the individual experiments are shown. For a summary of the parameters from several experiments, see Table 1. SP5 could not be accurately fit, although the heat changes observed indicate that the peptide does bind. A lower limit on the K_d is given, but no reliable parameters could be obtained.