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. 2016 Jul 8;291(35):18370–18383. doi: 10.1074/jbc.M115.713636

FIGURE 11.

FIGURE 11.

Effects of changing intracellular pH on ASIC1b/ASIC1a chimeras. A, schematic representation of ASIC1b (N1–42 aa)/1a (44 aa-C terminus) and ASIC1b (N1–52 aa)/1a (54 aa-C terminus) chimeric constructions. B, representative traces showing the effect of quinine on ASIC currents mediated by ASIC1b(N1–42 aa)/1a(44 aa-C terminus) chimera. Bath perfusion of 1 mm quinine still potentiated current amplitude mediated by ASIC1b(N1–42 aa)/1a(44 aa-C terminus) chimera (n = 7, t(13) = 0.5, unpaired t test, p > 0.05 compared with ASIC1a wild type, n = 8). C, representative traces and summary data showing the effect of quinine on current mediated by chimera ASIC1b (N1–52 aa)/1a (54 aa-C terminus) chimera. Quinine failed to potentiate current amplitude mediated by this mutant (n = 8, t(14) = 3.8, unpaired t test, **, p < 0.01 compared with ASIC1a wild type, n = 8). ns, no significant difference.