FIGURE 4.
Pyroptosomes of mutant caspase-1 spread during cell division. iBMDMcasp1/11-ko cells expressed both GFP-ASC and Ch-C284A. Pyroptosome formation was induced by priming with LPS (3 μg/ml) for 5 h and addition of nigericin (10 μm) for 1 h. Cherry channels (red) were merged with Hoechst (blue) and bright-field (gray) channels. Representative images from a time frame between 2 and 16 h after the addition of nigericin are provided. Arrowheads indicate a speck that divides into two (at 9:30) prior to cell division (starting at 14:30). The entire movie and further examples can be obtained in the supplemental material.