FIGURE 5.

RAP D1D2 K60A, K191A, and K60A/K191A mutations do not significantly alter the structure or stability of the molecule. CD spectroscopy was used to measure the molar ellipticity as an indicator of helical content at 20 °C (A) and 90 °C (B) for WT RAP D1D2 (black), RAP D1D2 K60A (blue), RAP D1D2 K191A (green), and RAP D1D2 K60A/K191A (purple). C, thermally induced denaturation of WT RAP D1D2 (black), RAP D1D2 K60A (blue), RAP D1D2 K191A (green), and RAP D1D2 K60A/K191A (purple) was measured using CD spectroscopy at 222 nm.