FIGURE 2.

Transcription analysis of collagen and collagen modifying enzymes. Real time PCR analysis was performed on GLT25D1-null (bars D1a and D1b), GLT25D2-null (bars D2), and control (bars C) cell lines with and without GLT25D1 (rGLT25D1) overexpression. Primers specific for GLT25D1 (A), GLT25D2 (B), PLOD3 (C), COL1A1 (D), and Col5A1 (E) were used. Statistically significant differences as determined by two-tailed Student's t test (p < 0.05) are marked by asterisks (n = 3 independent experiments).