TABLE 1.
Strains used in this study
| Strains | Genotypic description | Source |
|---|---|---|
| E. coli | ||
| DH5α | F− φ80dlacZΔM15Δ (lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rk−mk+) phoA supE44 λ−thi-1 gyrA96 relA1 | Invitrogen |
| BL21(DE3)pLysS | F− ompT hsdSB (rB−mB−) gal dcm (DE3) pLysS (CamR) | Invitrogen |
| S. cerevisiae | ||
| BY4741 | MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0 | Euroscarf |
| ddl1Δ | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YOR022c::kanMX4 | Euroscarf |
| cld1Δ | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YGR110w::kanMX4 | Euroscarf |
| gep4Δ | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YHR100c::kanMX4 | Euroscarf |
| crd1Δ | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YDL142c::kanMX4 | Euroscarf |
| taz1Δ | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YPR140w::kanMX4 | Euroscarf |
| aft1Δ | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YGL071w::kanMX4 | Euroscarf |
| aft2Δ | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YPL202c::kanMX4 | Euroscarf |
| ddl1Δtaz1Δ | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YPR140w::kanMX4; ddl1Δ::LEU2 | This study |
| cld1Δtaz1Δ | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YPR140w::kanMX4; cld1Δ::LEU2 | This study |
| cld1Δddl1Δ | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YGR110w::kanMX4; ddl1Δ::LEU2 | This study |
| RRY25 (cld1Δddl1Δtaz1Δ) | BY4741; Mat a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; YPR140w::kanMX4; ddl1Δ::LEU2; cld1Δ::HIS3 | This study |