TABLE 3.
Plasmids used in this study
| Plasmids | Construct description | Source |
|---|---|---|
| pYES2/NT B | Yeast expression vector with N-terminal His6 tag fusion | Invitrogen |
| pRSET A | E. coli expression vector with N-terminal His6 tag fusion | Invitrogen |
| YEp 351 | Yeast expression vector with leucine selection | ATCC |
| YEp 357 | Yeast episomal plasmids with lacZ reporter gene | ATCC |
| pUG34 | Yeast expression vector with histidine selection | Cathal Wilson |
| pRP1 | DDL1 gene is cloned into pYES2/NT B: pYES2/NT B-DDL1 (wild-type) | This study |
| pRP2 | Derived from pRP1 in which 1495GGC1497 is mutated to 1495GCT1497: pYES2/NT B-DDL1 (G1 to A) | This study |
| pRP3 | Derived from pRP1 in which 1501TCG1503is mutated to 1501GCT1503: pYES2/NT B-DDL1(S to A) | This study |
| pRP4 | Derived from pRP1 in which 1507GGT1509 is mutated to 1507GCT1509: pYES2/NT B-DDL1 (G2 to A) | This study |
| pRP5 | Truncated DDL1 gene is cloned into pYES2/NT B: pYES2/NT B-DDL1-DDHD (DDL1 gene devoid of C-terminal DDHD domain) | This study |
| pRP6 | PDDL1-lacZ reporter gene containing the DDL1 promoter into YEp357 | This study |
| pRP7 | AFT1 gene is cloned into pRSETA (pRSET A-AFT1) | This study |
| pRP8 | AFT2 gene is cloned into pRSETA (pRSET A-AFT2) | This study |
| BG1805 | Yeast expression vector with uracil selection | Open Biosystems |
| BG1805-AFT1 | AFT1 gene is cloned into BG1805 | Open Biosystems |
| BG1805-AFT2 | AFT2 gene is cloned into BG1805 | Open Biosystems |