Figure 3. Proteomics of male and female medaka livers.

Unscaled Venn’s diagram of the protein identified with at least 95% protein identification certainty in all of the 3 different 3-individual pools of male and/or female medaka livers (A). Volcano plot representation of the 1241 proteins according to female/male fold change average and significance of the differences (|log₂ FC| > 1.5 corresponding to |FC| > 3 and log₁₀ Pvalue < 2 corresponding to Pvalue < 0.01) determined according to Scaffold 4.5.1 semi-quantitative values based on both MS and MS/MS data (B). Female and male over-represented proteins are determined with positive and negative significant FC (F/M) values and are shown in red and blue, respectively. Top-25 lists of the male- and female-superabundant proteins (C and D, respectively).