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The relationship between the minor allele frequency (maf) threshold (x-axis) and the total number of variable sites (y-axis) detected by [7]. Lowering the MAF threshold leads to an exponential increase in the number of variable positions. The image was generated by applying variable MAF thresholds to data from 156 human samples and plotting the average number of variable sites at a given MAF threshold. The line thickness corresponds to the 95 % confidence interval around the mean value
