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. 2016 Aug 26;17(1):180. doi: 10.1186/s13059-016-1039-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The Du Novo approach. First, reads tagged with identical barcodes are grouped into strand-specific families. Reads within each family are aligned and single-stranded consensus sequences (SSCSs) are generated. Finally, the SSCSs are reduced into duplex consensus sequences (DCSs)