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. 2016 Aug 26;16(1):449. doi: 10.1186/s12879-016-1793-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Biofilm formed by living and dead cells of Haemophilus parainfluenzae SHpiNP18C strain. Expanations: Biofilm formed by living (a, b) and dead (c, d) cells of Haemophilus parainfluenzae SHpiNP18C and detected using two-dimensional (2d: a, c) or three-dimensional (3d: b, d) CLSM image with gray level values varying from 0 to 250 (b, d) after 24 h of incubation. The layer of living cells was located on or between the structures formed by dead cells. The living and dead cells forming biofilm were detected in the green and red canals, respectively, and the structure of the biofilm was shown in XZ plan on the basis of 3D scan