Table 6.
Distribution of Haemophilus influenzae and Haemophilus parainfluenzae biofilm-producers in patients with sarcoidosis and in healthy people
| Category of biofilm producers | OD570 range | No. (%) of isolates | |||
|---|---|---|---|---|---|
| Haemophilus influenzae | Haemophilus parainfluenzae | ||||
| Healthy people | |||||
| Nasopharynx (n = 1) | Nasopharynx (n = 65) | ||||
| Non-producers | 0.0 | 0 (0.0) | 8 (12.3) | ||
| Weak | 0.01–0.24 | 1 (100) | 15 (23.1) | ||
| Moderate | 0.25–0.48 | 0 (0.0) | 3 (4.6) | ||
| Strong | 0.49–0.96 | 0 (0.0) | 12 (18.5) | ||
| Very strong | ≥0.97 | 0 (0.0) | 27 (41.5) | ||
| Patients with sarcoidosis | |||||
| Nasopharynx (n = 4) | Sputum (n = 8) | Nasopharynx (n = 31) | Sputum (n = 26) | ||
| Non-producers | 0.0 | 1 (25.0) | 3 (37.5) | 12 (38.7) | 6 (23.1) |
| Weak | 0.01–0.24 | 3 (75.0) | 5 (62.5) | 11 (35.5) | 9 (34.6) |
| Moderate | 0.25–0.48 | 0 (0.0) | 0 (0.0) | 0 (0.0) | 3 (11.5) |
| Strong | 0.49–0.96 | 0 (0.0) | 0 (0.0) | 1 (3.2) | 4 (15.4) |
| Very strong | ≥0.97 | 0 (0.0) | 0 (0.0) | 7 (22.6) | 4 (15.4) |
Distribution of biofilm-producers was detected using the crystal-violet (CV) method. The cut-off OD (ODc; here: 0.1 ± 0.02) was defined as three standard deviations above the mean OD570 of the negative control. The categories of biofilm-producers: 0 - non-producers (OD ≤ ODc); 1, weak (ODc < OD ≤ 2xODc); 2, moderate (2xODc < OD ≤ 4xODc); 3, strong (4xODc < OD ≤ 8xODc); 4, very strong (8xODc < OD) producers, according to [26]