Table 2.
Identification of host-derived proteins with identical tryptic peptides to I. scapularis tick homologues
| ID | Description | Unique host-derived peptides |
|---|---|---|
| P31081 | HSP60 | ALMLQGVDLLADAVAVTMGPK |
| VGGTSDVEVNEK | ||
| VGGTSDVEVNEKKDR | ||
| P0CB32 | HSP70 | FDLTGIPPAPR |
| RKELEQVCNPIITK | ||
| P68432 | Histone H3.1 | RVTIMPKDIQLAR |
| SAPATGGVK | ||
| SAPATGGVKKPHRYRPGTVALR | ||
| F1MN93 | TOP1 uncharacterized protein | AGNEKEEGETADTVGCCSLR |
| HLQDLMEGLTAK | ||
| P62808 | Histone H2B type 1 | AMGIMNSFVNDIFER |
| EIQTAVRLLLPGELAK | ||
| EIQTAVR | ||
| ESYSVYVYK | ||
| SRKESYSVYVYK | ||
| STITSREIQTAVRLLLPGELAK | ||
| STITSREIQTAVR | ||
| VLKQVHPDTGISSK | ||
| W5PLV3 | RAB5B uncharacterized protein | TAMNVNDLFLAIAK |
| P62803 | Histone H4 | None |
| Q9TTS3 | Acetyl-CoA carboxylase 1 | None |
To confirm the origin for selected differentially represented proteins (Table 1), all of the peptides used to identify the proteins sharing tryptic peptides with I. scapularis proteins were revised. The peptides unique for host-derived proteins are shown. For protein P62803, we could not define the origin due to 100 % homology between sheep and tick proteins. For protein Q9TTS3, all peptides used for identification were identical in both host and tick-derived proteins