Table 3.
Differential expression/representation of enzymes involved in tick hemoglobinolytic and heme transport pathways in response to A. phagocytophilum infection
| ID | Description | Log2 (infected/uninfected) fold change (mRNA/protein) | Role in hemoglobin digestion | |
|---|---|---|---|---|
| Midguts | Salivary glands | |||
| Tick hemoglobinolytic pathway | ||||
| EF428204 | Cathepsin D | nf/ns | ns/nf | Primary cleavage |
| A4GTA5 | ||||
| HQ615697 | Cathepsin D2 | nf/ns | ns/nf | Primary cleavage |
| E7E820 | ||||
| ISCW000202 | Legumain | -0.6/ns | ns/ns | Primary cleavage |
| B7P6S9 | ||||
| ISCW015983 | Legumain | -1.3/nf | nf/nf | Primary cleavage |
| B7P2C6 | ||||
| ISCW000076 | Cathepsin L | -7.3/nf | nf/nf | Primary and secondary cleavage |
| B7P3N8 | ||||
| JX502821 | Cathepsin L | nf/nf | nf/-2.9 | Primary and secondary cleavage |
| J9QSA1 | ||||
| ISCW000080 | Cathepsin B | +0.5/ns | nf/-2.1 | Secondary and tertiary cleavage |
| B7P3P1 | ||||
| EU551624 | Cathepsin B | nf/ns | nf/-2.0 | Secondary and tertiary cleavage |
| B7SP39 | ||||
| ISCW013346 | Cathepsin B | -1.6/ns | ns/-2.5 | Secondary and tertiary cleavage |
| B7QCU7 | ||||
| ISCW000078 | Cathepsin B | +0.2/ns | -4.3/-1.8 | Secondary and tertiary cleavage |
| B7P3P0 | ||||
| ISCW003494 | Cathepsin C | +0.3/ns | -2.6/+2.2 | Tertiary cleavage |
| B7PEB4 | ||||
| ISCW001779 | Leucine aminopeptidase | +2.2/ns | ns/nf | Tertiary cleavage |
| B7P2N4 | ||||
| ISCW023735 | Leucine aminopeptidase | +2.0/ns | +1.1/ns | Tertiary cleavage |
| B7QLQ7 | ||||
| ISCW001780 | Leucine aminopeptidase | +1.4/ns | +0.5/ns | Tertiary cleavage |
| B7P2N5 | ||||
| ISCW013904 | Serine carboxipeptidase | +1.0/ns | ns/ns | Tertiary cleavage |
| B7QLB7 | ||||
| ISCW024536 | Serine carboxipeptidase | -0.5/ns | ns/nf | Tertiary cleavage |
| B7Q049 | ||||
| ISCW024751 | Serine carboxipeptidase | -0.7/ns | ns/ns | Tertiary cleavage |
| B7QD81 | ||||
| ISCW024883 | Serine carboxipeptidase | -4.5/nf | -2.4/nf | Tertiary cleavage |
| B7QK83 | ||||
| ISCW007492 | Serine carboxipeptidase | -1.7/ns | ns/ns | Tertiary cleavage |
| B7PTE5 | ||||
| ISCW003059 | Serine carboxipeptidase | -1.2/nf | ns/nf | Tertiary cleavage |
| B7PC00 | ||||
| Tick heme transport pathway | ||||
| ISCW001847 | Heme-responsive gene 1 (HRG1) | -0.8/nf | ns/nf | Heme transporter |
| B7P8M4 | ||||
| ISCW021709 | Heme-binding lipoprotein (HELP) | +3.3/ns | -0.2/ns | Heme transporter |
| B7Q406 | ||||
| ISCW013727 | Vitellogenin 1 (VG1) | ns/ns | ns/nf | Heme transporter |
| B7QJ67 | ||||
| ISCW021228 | Vitellogenin 2 (VG2) | -1.1/nf | ns/nf | Heme transporter |
| B7Q7E5 | ||||
Transcriptomics RNAseq and proteomics data from A. phagocytophilum-infected and uninfected tick samples were obtained from Ayllón et al. [12]. Except for Cathepsins D and D2, which were included to show that these proteins were identified in the proteomics analysis but were not significantly different between infected and uninfected samples, only genes/proteins with statistically significant differences in at least one of the analyses (transcriptomics or proteomics) and samples (midguts or salivary glands) were included. Abbreviations: ID, gene (GenBank; http://www.ncbi.nlm.nih.gov) and protein (Uniprot; http://www.uniprot.org) accession numbers
+, upregulated/overrepresented genes/proteins in infected vs uninfected ticks; -, downregulated/underrepresented genes/proteins in infected vs uninfected ticks; nf, not found; ns, not significant