Table 1.
Primer pairs used for amplification of rickettsial genes
| Target gene, primer pair no., primer name | Specifity | Sequence | Amplified fragment (nt) | Reference | |
|---|---|---|---|---|---|
| gltA | Genus Rickettsia | ||||
| 1 | CS-78 | 5'-GCAAGTATCGGTGAGGATGTAAT-3' | 401 | [19] | |
| CS-323 | 5'-GCTTCCTTAAAATTCAATAAATCAGGAT-3' | [19] | |||
| 2 | CS-239 | 5'-GCTCTTCTCATCCTATGGCTATTAT-3' | 834 | [38] | |
| CS-1069 | 5'-CAGGGTCTTCGTGCATTTCTT-3' | [38] | |||
| ompA | Spotted fever group (SFG) | ||||
| 3 | Rr190.70p | 5'-ATGGCGAATATTTCTCCAAAA-3' | 632 | [39] | |
| 190-701 | 5'-GTTCCGTTAATGGCAGCATCT-3' | [40] | |||
| ompB | Genus Rickettsia a | ||||
| 4 | 120-M59 | 5'-CCGCAGGGTTGGTAACTGC-3' | 820 | [41] | |
| 120-807 | 5'-CCTTTTAGATTACCGCCTAA-3' | [41] | |||
| htrA | Genus Rickettsia | ||||
| 5 | 17k-5 | 5'-GCTTTACAAAATTCTAAAAACCATATA-3' | 549 | [38] | |
| 17k-3 | 5'-TGTCTATCAATTCACAACTTGCC-3' | [38] | |||
aExcept for some species of basal groups (e.g. Rickettsia bellii)