Fig. 6.

Effect of selected inhibitors on adipogenic differentiation of hMSCs. a Effect of Batimastat, added at the indicated concentrations to hMSCs in adipogenic differentiation medium, on adipogenic (upper panel; Oil Red O (ORO) staining, day 9) and osteogenic differentiation (lower panel; alkaline phosphatase (ALP) staining, day 7). No addition (Cont), TGFβ (2 ng/ml), and DMSO (0.1 %; used as a solvent for Batimastat) were used as controls. b Inhibition of adipogenic differentiation by Batimastat (Bat) and Zopolrestat (Zop), added at the indicated micromolar concentrations. Quantitative Oil Red O staining was measured at 530 nm on hMSCs treated for 9 days with adipogenic differentiation medium. TGFβ (5 ng/ml) was used as a control. Significance levels are indicated relative to untreated controls (Cont). *p < 0.05; **p < 0.01; ***p < 0.001. DMSO dimethyl sulfoxide, TGFβ transforming growth factor beta