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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Cancer Cell. 2016 Aug 8;30(2):197–213. doi: 10.1016/j.ccell.2016.07.006

Figure 6. CBX8 Recruits BCOR Complex to H3K27me3 Marked Bivalent Genes and Is Required for the Biological Actions of EZH2.

Figure 6

(A) Expression level in FPKM (fragments per kilobase of transcript per million mapped reads) of CBX genes in four naive B cells (NB) and four germinal center B cells (GCB) samples. Values are means ± SEM.

(B) RT-qPCR of CBX8 in four NB and four GCB samples. Values are means ± SEM.

(C) Immunoblotting of whole-cell lysates from NB and GCB cell samples.

(D) Myc immunoblotting of Flag IPs using whole-cell extracts from Sf9 insect cells transfected with expression vectors for the indicated proteins, m, amino terminal myc epitope tag; f, flag tag; mf, both tags.

(E) Immunoblotting of immunoprecipitation (IP) using whole-cell lysates from the indicated cell lines.

(F) Immunoblotting of IP using whole-cell lysates from primary GCB from human tonsils.

(G) CBX8 qChIP in OCI-Ly7 cells treated with 2 µM GSK343 or GSK669 for 72 hr. qPCR was performed using primers targeting the promoters of the indicated genes.

(H) Immunoblotting of whole-cell lysates from OCI-Ly7 cells expressing two independent CBX8 shRNAs or control.

(I) BCOR and H2AK119ub qChIP was done in cells from (H).

Values in (G) and (I) are shown as means of triplicates or quadruplicates ±SD. t test, *p < 0.05, **p < 0.01, ***p < 0.001. See also Figure S6, Tables S2, and S3.