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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Jun 14;1861(9 Pt A):1076–1082. doi: 10.1016/j.bbalip.2016.06.006

Fig. 1.

Fig. 1

Analysis by 2D-TLC of lipids from: A) R. lituseburensis, B) R. ilealis, and C) strain FRIFI. The upper plates were stained with ninhydrin and after recording, with molybdenum blue followed by charring and the lower plates were stained with α-naphthol. The major lipids were identified by their staining properties and confirmed by LC/MS as follows: PL1, PG; PL2, CL; PL3, PG; PL4, lysyl-PG, and PL5, PA. The glycolipids were initially shown by staining with α-naphthol and their identities revealed by LC/MS as follows: GL1, MHDAG, GL2, DHDAG; GL3, acyl-TriHDAG, GL4, TriHDAG; GL5, acyl-TetraHDAG; and GL6, TetraHDAG. Lysyl-PG was ninhydrin-positive. Standards of PG and CL were applied to the left margin and in the upper right hand corner, upper plates and a standard of MHDAG was applied to the left margin and upper right hand corner of the lower plates stained with α-naphthol. The resulting spots are shown with arrows.