Identification of lncRNAs expressed in B cells. (A)
Schematic representation of the ontogenetic relationships between B-cell
populations used to generate the lncRNA catalog. Solid arrows indicate
developmental progression through B-cell stages or activation (to GC B
cells). Dashed line indicates recirculation of follicular B cells back
to the bone marrow. B1A, B1a B cells; FO, follicular B cells; GC,
germinal center B cells; IMM, immature B cells; MAT, mature B cells; MZ,
marginal zone B cells; PRE, pre-B cells ; PRO, pro-B cells. (B) Genomic
distribution of the 1491 multiexon and 3025 single-exon lncRNAs
identified by this study. Positions are described relative to Ensembl
v72 protein-coding gene annotations as antisense (overlapping a coding
gene on antisense strand), flanking (<5 kb from coding gene), and
intergenic (>5 kb from coding gene). (C) Overlap between the 2349
intergenic lncRNAs identified by this study (B cell), with those
identified in T lineage cells18 (T cell), and those annotated in Ensembl v78.
Kernel density plots representing the distribution of distance between
each multiexon (D) and single-exon (E) intergenic lncRNA TSS and the
nearest annotated TSS on the same strand that appeared in 2 or more of
the 128 mouse cell lines considered by the FANTOM5 consortium. CAGE, cap
analysis of gene expression. Shaded regions indicate a null distribution
as measured by distance to the nearest FANTOM5 annotated TSS on the
opposing strand. Vertical gray dashed line indicates a distance of 500
bp. (F) Coverage of the genome and of lncRNA exons by the indicated
transposon elements.