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. 2016 Jul 25;17(8):1197. doi: 10.3390/ijms17081197

Table 4.

Accessions of genes and primers sequences used for real-time qPCR. The exact sequence of each MtPIN first exon fragment that was used for the primer design is also shown (primers binding sites are marked by shading). Underlines represent start codon of each PIN gene and dots (…) represent the discontinuity within the sequence. bp: base pairs.

Gene/ID Forward (F) and Reverse (R) Primer Sequence Sequence of Each MtPIN First Exon Fragment Product Length
5′–3′ (bp)
MtPIN1 (MTR_4g084870) F: TCCACTTTACGTAGCCATGATCT ATGATAACCTGGCACGATCTATACACAGTTTTAACCGCAGTAGTTCCACTTTACGTAGCCATGATCTTAGCCTATGGCTCCGTACGGTGGTGGAAAATATTCTCACCGGACCAATGTTCCGGCATAAACCGTTTCGTCGC 74
R: AACATTGGTCCGGTGAGAAT
MtPIN2 (MTR_4g127100) F: CGAAGATGAGACATTGAGGATG ATGATTACCGGTAAGGATATATACGATGTTTTCGCA...CGAAGATGAGACATTGAGGATGCATAAGAAAAGGGGAGGGAGGAGTATGAGTGGTGAGTTGTTCAATAATGGTGGTTCTTACCCTCCTCCAAATCCTATGC 74
R: CACCATTATTGAACAACTCACCA
MtPIN3 (MTR_1g030890) F: CTGGCCTCAACGTGTTCC ATGATAACACTAAAAGATCTTTACACTGTCTTAACAGCAGTGGTTCCA...CTGGCCTCAACGTGTTCCGAAATTCGGAACAATCGGAAGAGGGTGCTAAGGAGATCAGGATGGTGGTGGCTGATGAACATAATCAAAA 68
R: CACCACCATCCTGATCTCCT
MtPIN4 (MTR_6g069510) F: TGGTGCCACTTTATGTAGCTATG ATGATCACTTTAACAGATTTCTACCATGTCATGACAGCAATGGTGCCACTTTATGTAGCTATGATCTTAGCTTATGGATCAGTAAAATGGTGGAAAATATTTTCACCTGATCAATGTTCAGGAATCAACCGTTTTGTTGCA 92
R: ACGGTTGATTCCTGAACATTG
MtPIN5 (MTR_8g107360) F: CGTGGCTATGATATTAGCTTATGG ATGATAACGTTAACAGATTTCTACCATGTGATGACATCAATGGTGCCACTTTACGTGGCTATGATATTAGCTTATGGTTCAGTGAAATGGTGGAAGATATTCTCTCCCGATCAATGCTCCGGCATCAATCGCTTCGTTGCT 66
R: GAGCATTGATCGGGAGAGAA
MtPIN6 (MTR_1g029190) F: TAAACCGATTCGTCGCAGTT ATGGTGACAAGAGAAGATTTATACAACGTGATGTGTGCAATGGTACCTC...TAAACCGATTCGTCGCAGTTTTTGCTGTTCCAGTTCTATCTTTTCACTTCATTTCTCTCAACAATCCTTATCAAATGGACACAAAATTTAT 67
R: GGATTGTTGAGAGAAATGAAGTGA
MtPIN7 (MTR_4g127090) F: TTGTGCCACTATATGTCGCTATG ATGATTACCGGCAAGGACATATACAATGTTTTAGCGGCGATTGTGCCACTATATGTCGCTATGATATTAGCATATGGTTCGGTCCGATGGTGGAAAATCTTCACACCAGATCAATGTTCTGGAATAAACCGTTTTGTCTC 94
R: AAACGGTTTATTCCAGAACATTG
MtPIN8 (MTR_7g009370) F: TTTCCTTAGCCAATGTTTATCATGT ATGATTTCCTTAGCCAATGTTTATCATGTAATAACAACAACTGTCCCATTATATGTAACAATGATACTAGCCTATGTCTCAGTGAAATGGTTTAAGATCTTCACACAAGAACAATGTTCAGGAATAAACAAATTTGTTGC 95
R: GATCTTAAACCATTTCACTGAGACATA
MtPIN9 (MTR_7g079720) F: AGCAGTGGTGCCACTCTATTTT ATGATTGGGTGGGAAGACGTGTACAAAGTTATTGTAGCAGTGGTGCCACTCTATTTTGCACTAATATTAGGCTATGGTTCTGTAAGGTGGTGGAAAATTTTCACAAGAGAACAATGTGATGCAATAAACAAACTAGTTT 96
R: TTGTTTATTGCATCACATTGTTCTC
MtPIN10 (MTR_7g089360) F: TGGTGTTGCTAAAGCTAATGGA ATGATAAGTGCTTTAGACTTATACCATGTCCTCACAGCAGTAGTACC...TGGTGTTGCTAAAGCTAATGGAAATGGTGGAAATGGCTACCCTGCTCCTCATAGTGCAGGGATTTTTTCACCTGTGGCTAATAAGAAAAA 61
R: CCCTGCACTATGAGGAGCA
MtPIN11 (MTR_6g011400) F: ACAGCCACTGTCCCATTATATGT ATGATTTCCTTAATTGATGTCTATCATGTAGTAACAGCCACTGTCCCATTATATGTAACTATGTTACTAGCATACATTTGTGTTAAATGGTGGAAACTTTTCACACCAGATCAATGTGCAGGCATAAACAAATTTGTAGC 87
R: TGCACATTGATCTGGTGTGA
MtRPS7b (MTR_8g087480) F: GAAACAACACTGCAATTTACAGGA ATGACCTTACCATACCCATACCATCACCATTGTTGT...GAAACAACACTGCAATTTACAGGAAACTATCAGGCAAAGATGTTGTCTTTAGTATCCCGTTACTGAGGCTTAGGTTCTGTTTCTCAATTTTGATTTTGTTTCATG 74
R: CCTAAGCCTCAGTAACGGGATA