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. 2016 Jul 28;17(8):1206. doi: 10.3390/ijms17081206

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunoassay specificity of the monoclonal antibody against OaLypla1. (A) Immunoassay specificity against the recombinant OaLypla1. M: protein marker (Thermo, Waltham, MA, USA); lane 1: total proteins from the uninduced pET-30a-LYPLA1-W cells; lane 2: total proteins from the induced pET-30a-LYPLA1-W cells; lane 3: total proteins from the induced pET-30a-LYPLA1-H cells; (B) Immunoassay specificity against the native OaLypla1. M: protein marker (Thermo, Waltham, MA, USA); lane 1: total proteins from the induced pET-30a-LYPLA1-H cells as a positive control; lane 2: total proteins extracted from the kidney of O. aries. The pET-30a-LYPLA1-W cells were induced to express the recombinant OaLypla1 with no His₆-tag, and the pET-30a-LYPLA1-H cells expressed OaLypla1 fused with a His₆-tag.