Table 2.
Characterization of GST genes in Brassica oleracea.
| Sr. No. | Class | No. of GST Genes | Nucleotide Length Range (bp) | ORF Range (bp) | No. of Exons | Protein | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Length Range (aa) | Mol. Wt. Range (kDa) | pI Range | Average Domain Range | ||||||||
| GST N-Region | GST C-Region | EF1G Region | |||||||||
| 1 | Tau | 28 | 705–4093 | 570–942 | 1–2 | 189–313 | 21.56–35.13 | 4.96–8.85 | 72–75 | 110–146 | - |
| 2 | Phi | 14 | 780–1198 | 603–777 | 3 | 200–258 | 22.59–28.84 | 5.13–8.21 | 60–75 | 114–118 | - |
| 3 | Theta | 2 | 1406–1470 | 726–738 | 7 | 241–245 | 27.38–27.66 | 9.36–9.5 | 75 | 128 | - |
| 4 | Zeta | 2 | 1994–2097 | 591–714 | 9 | 196–237 | 22.26–26.35 | 5.29–6.91 | 44–77 | 118–119 | - |
| 5 | Lambda | 3 | 1430–1776 | 708–906 | 8–9 | 187–301 | 21.37–34.39 | 5.08–8.82 | 77–68 | 88–121 | - |
| 6 | DHAR | 4 | 851–1421 | 633–774 | 3–6 | 210–257 | 23.22–28.63 | 5.76–8.28 | 56–72 | 118–121 | - |
| 7 | TCHQD | 1 | 1071 | 801 | 2 | 266 | 31.46 | 9.26 | 72 | 99 | - |
| 8 | EF1G | 3 | 1902–2326 | 1239–1248 | 6–7 | 412–415 | 46.4–46.57 | 5.56–5.64 | 71–81 | 107–120 | 106–108 |
| 9 | GHR | 5 | 1290–1576 | 954–1212 | 3–5 | 317–403 | 36.43–44.9 | 6.32–8.2 | 88–106 | 111–141 | - |
| 10 | GST2N | 2 | 2196–2401 | 1011–1017 | 11–12 | 336–338 | 36.93–36.95 | 8.81–9.26 | 76–77 | - | - |
| 11 | mPGES2 | 1 | 1464 | 942 | 6 | 313 | 35.13 | 8..85 | 72 | 146 | - |
Sr. No., serial number; bp, base pair; Mol. Wt., molecular weight; aa, amino acid; kDa, Kilodalton; pI, Iso-electric point; GST, Glutathione transferase; N-, N-terminal; C-, C-terminal; EF1G, Elongation factor 1 γ.