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. 2016 Jul 27;17(8):1217. doi: 10.3390/ijms17081217

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Ultra high performance liquid chromatography coupled to high resolution tandem mass spectrometry base peak chromatograms of ancient wheat extract obtained using positive (A) and negative (B) ionization modes. Extracted ion chromatogram (XIC) of Lyso PC 16:0 ionized in positive ([M + H]⁺ m/z 496.3399) (C) and negative ([M + HCOO]⁻ m/z 540.3332) (D) modes. Product ions acquired automatically by the information-dependent acquisition (IDA) method for the m/z 496.3399 (E) and m/z 540.3332 (F) parent ions. Blue arrows are thresholds and indicators in terms of RT and m/z values.