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. 2016 Jul 30;17(8):1237. doi: 10.3390/ijms17081237

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of VEGF and FGF2 on the rate of metaphase II (MII) oocyte, fertilization, and blastocyst development. Mice (n = 24) grafted with ovarian tissue three weeks after transplantation were treated with 150 IU gonadotropins, and MII oocytes were collected from the antral follicles for in vitro fertilization (IVF). Fertilization oocytes were cultured to the blastocyst stage. (A) The rate of MII oocytes; (B) fertilization rate; and (C) the rate of blastocyst formation. These results were statistically analyzed using the relative percentage data shown in Table S3. Values are the mean ± SD of six independent experiments (n = 4 each). * p < 0.05 compared with the control group.