Figure 4.

Deletion analysis of hST8Sia VI promoter in SK-N-BE(2)-C cells with physcion treatment. Reporter plasmids contain progressive deletions from the 5′ end (A) and the 3′ end (B) of the hST8Sia VI gene promoter. After co-transfection of each construct into SK-N-BE(2)-C cells with pRL-TK, relative firefly luciferase (Luc) activity was assessed. The pRL-TK-derived Renilla luciferase activity was used to normalize all firefly luciferase activity. Data are the means ± SD of triplicate measurements and are representative of three independent experiments.