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. 2016 Aug 2;17(8):1246. doi: 10.3390/ijms17081246

Figure 6.

Figure 6

Physcion induced transcriptional activation of hST8Sia VI through ERK/p-38 MAPK signal pathways in SK-N-BE(2)-C cells. For representative immunoblot analysis of p-ERK, ERK, p-JNK, JNK, p-p38 and p38 protein expression levels, cells were treated with 40 μM physcion for different lengths of time (A). pGL3-574 or pGL3-Basic was co-transfected into SK-N-BE(2)-C cells with pRL-TK. The transfected cells were incubated for 24 h in the presence of 40 μM physcion with different inhibitors (10 μM U0126, 10 μM SP600125, and 20 μM SB203580); The Renilla luciferase activity derived from pRL-TK was used to normalize all firefly luciferase activity. Data are the means ± SD of triplicate measurements and are representative of three independent experiments (B). +, treatment; -, untreatment.