Table 1.

Primer sequences used for reverse transcription-polymerase chain reaction (RT-PCR) and deletion constructs, site-directed mutagenesis and chromatin immunoprecipitation (ChIP).

Primer	Sequence	Strand	Purpose
hST8Sia VI	5′-CCCTATTTCTGGAGGACATTGCAACCTA-3′	Sense	RT-PCR
hST8Sia VI	5′-GTTGGAGGATCTGGCTGTATTCTTTG-3′	Antisense	RT-PCR
β-actin	5′-CAAGAGATGGCCACGGCTGCT-3′	Sense	RT-PCR
β-actin	5′-TCCTTCTGCATCCTGTCGGCA-3′	Antisense	RT-PCR
GAPDH	5′-AGCCTCAAGATCATCAGCAATGTCCT-3′	Sense	RT-PCR
GAPDH	5′-AAATTCGTTGTCATACCAGGAAATGAG-3′	Antisense	RT-PCR
P-2660	5′-ATGGTACCCTTCTGCTGTTGCCTTGAGCCCAGC-3′	Sense	Deletion
P-2660	5′-ATCTCGAGACAGCGTTCACAGGCGGCAGCGAG-3′	Antisense	Deletion
P-1982	5′-ATGGTACCGGCTGTCTGGCCTGGTTGCTCCCA-3′	Sense	Deletion
P-1503	5′-ATGGTACCAAGGATACCATAGGCTGGGTGACCG-3′	Sense	Deletion
P-968	5′-ATGGTACCAGGCTGCCTTGTGGGGCCTGGTATA-3′	Sense	Deletion
P-574	5′-ATGGTACCGCCCCTCATACCAGTTCGCTGTCCC-3′	Sense	Deletion
P-240	5′-ATGGTACCCGCGCGGCGGCGGCGGCAGCAGC-3′	Sense	Deletion
P-320A	5′-ATCTCGAGTTCTGCGCCCTCGCCTCGTCCCGA-3′	Antisense	Deletion
P-630A	5′-ATCTCGAGCCTGGAGACCCGTTTAGCCCCTG-3′	Antisense	Deletion
P-1066A	5′-ATCTCGAGGGGTGGACCTCATGGACCTCCTC-3′	Antisense	Deletion
Pax-5 Mut	5′-GGAGTTGAGCTTCCGCATTCCAACCTTCAGGTGACC-3′	Sense	Mutagenesis
Pax-5 Mut	5′-GGTCACCTGAAGGTTGGAATGCGGAAGCTCAACTCC-3′	Antisense	Mutagenesis
NF-Y Mut	5′-GCAGAAAACTTGGAGCAATCAGCACGGAGTTGAGC-3′	Sense	Mutagenesis
NF-Y Mut	5′-GCTCAACTCCGTGCTGATTGCTCCAAGTTTTCTGC-3′	Antisense	Mutagenesis
hST8Sia VI	5′-AGGCAGAGTTGTGGTGTGGC-3′	Sense	ChIP
hST8Sia VI	5′-TGGCAGATGACGATTCGCCGA-3′	Antisense	ChIP

Primers P-2660S to P-240S were used for construction of the deletion mutants. KpnI and XhoI sites in sense and antisense primers, respectively, are underlined. The mutated nucleotides in the oligonucleotides for mutation are in boldface and italic type.