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Detection of a series of diluted B. burgdorferi DNA by basic recombinase polymerase amplification (B-RPA) and LF-RPA. (A) The B-RPA results shown by agarose gel electrophoresis. M stands for marker, N stands for negative control (water); (B) The LF-RPA results shown by Hybridetect 2T dipsticks. N stands for negative control (water); (C) Detection of B. burgdorferi DNA from 104 copies to one copy by LF-RPA assay.
