Figure 1.

Flow chart of the drought treatments and quantitative proteomics procedure. Plants were grown in a “sandwich” system (A); During the 20th day of the water withholding period, physiological data were recorded on both drought-treated (B); and well-watered control plants (C). Leaf protein samples were extracted followed by the ProteoMiner enrichment. Quantitative proteomics analysis was performed using the crude leaf protein extracts and the ProteoMiner-enriched samples. Functional pathways were developed using information on the drought-induced changes in the leaf proteomes, and the association between protein expression and physiological properties was developed focusing on drought stress tolerance.