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. 2016 Aug 3;17(8):1256. doi: 10.3390/ijms17081256

Figure 2.

Figure 2

Effect of indole-3-carbinol (I3C) on lipid accumulation in differentiated adipocytes. Cells were treated with various concentrations of I3C for 24 h, and the cells were stained with oil red O. Intracellular oil red O and triglyceride (TG) (A) and extracellular glycerol (B) contents were quantified using the method described in the Materials and Methods. Values are the mean ± SD from three measurements. Bars with different letters (a–d) or different symbols (+, #, *) significantly differ from each other (p < 0.05).