Figure 4.

pDCs response to TLR7 stimulation is not affected in young and old NZW mice. Purified BM-derived pDCs from NZW mice age-matched with the pre-symptomatic (A, shown as Young) and symptomatic (B, shown as Old) NZB/W NZB/W F1 mice were treated with (+) or without (−) 5 µg/mL of R837 for 48 h. Expression of MHC class II, CD40 and CD86 were examined and shown as representative histograms in (A). Grey: Isotype; dotted line: unstimulated; solid line: R837 activated. Collective data illustrating changes in the mean fluorescence intensity (MFI) of MHC class II or percentages (%) of CD40 and CD86 are shown in (B). Each symbol represents one experimental mouse, n = 5. Data were collected from at least three independent experiments. Bar: mean value.