Figure 2.
OLE modulates the expression of Bax/Bcl-2 ratio. CTRL and OLE conditions do not show modulation of the pro-apoptotic Bax/anti-apoptotic Bcl-2 ratio. Treatment with 40 µM 6-OHDA for 24 h significantly increases the Bax/Bcl-2 ratio whereas a 3 h pre-treatment with 10−12 M OLE prevents this increment. Bottom: Bax and Bcl-2 bands, as revealed by Western blotting. Bax and Bcl-2 optical densities were measured on the same membrane. Ratios were performed between Bax and Bcl-2 of the same condition. Data are expressed as means ± SEM, n = 3. *** p < 0.001 compared with CTRL; and + p < 0.05 compared with 6-OHDA.