Table 1.
HPLC–DAD-MS/MS characterisation of main polyphenols present in crude ethanolic leaf extract (CEE) of C. incanus. Compounds numbers correspond to those indicated in Figure 1. (n.d *, not detected; sh, shoulder).
| Peak n | tR (min) | Λ (nm) | [M-H]− (m/z) | MS2 (m/z) | Tentative Assignement |
|---|---|---|---|---|---|
| 1 | 4.6 | 234,270 | 331 | 125, 169 | Monogalloyl glucose |
| 2 | 8.3 | 234,272 | 169 | 125 | Gallic acid |
| 3 | 16.9 | 236,272 | 609 | 441, 423, 483, 305, 303 | (Epi)Gallocatechin dimer |
| 4, 5 | 19.5 | 234,272 | 305 | 611, 125, 137 | (−)-Gallocatechin and (−)-epigallocatechin |
| 6 | 20.6 | 236,276 | 593 | 407, 467, 425, 289, 285 | (Epi)gallocatechin-(epi)catechin or (Epi)catechin-(epi)gallocatechin |
| 7, 8 | 21.5 | 236,278 | 289 | 245, 205 | (+) Catechin and (−) Epicatechin |
| 9 | 24.2 | 260,360 | n.d * | - | Myricetin derivative 1 |
| 10 | 24.5 | 254,362 | 479 | 316, 271 | Myricetin-3-O-hexoside |
| 11 | 25.4 | 260,360 | n.d * | - | Myricetin derivative 2 |
| 12 | 25.6 | 260,358 | 463 | 316, 271, 179 | Myricitrin |
| 13 | 25.7 | 256,356 | 609 | 301 | Rutin |
| 14 | 26.6 | 265,355 | 433 | 301, 271 | Quercetin-3-O-pentoside |
| 15 | 26.9 | 256,350 | 447 | 301, 179 | Quercitrin |
| 16 | 27.8 | 264,352 | n.d * | - | Quercetin derivative 1 |
| 17 | 28.2 | 264,352 | n.d * | - | Quercetin derivative 2 |
| 18 | 29.5 | 264,314,346sh | 593 | 285, 145 | Kaempferol 3-O-rutinoside |
| 19 | 33.3 | 268,314,348sh | 739 | 285, 306, 145, 452 | Kaempferol-3-(3″,6″-dicoumaroyl)-glucose |