Table 2.
Primer sequences for reverse transcription PCR.
| Gene Code | Accession ID | Sequence (5′→3′) | Temperature (°C) | Product Length (bp) | |
|---|---|---|---|---|---|
| MMP-1 | NM_001145938.1 | Forward | AGTGACTGGGAAACCAGATGA | 57 | 159 |
| Reverse | CGTCTTGGCAAATCTGGCCTGTAA | ||||
| MMP-2 | NM_001127891.2 | Forward | GCAGTGGGGGCTTAAGAAGA | 57 | 969 |
| Reverse | AGCCGTACTTGCCATCCTTC | ||||
| MMP-3 | NM_002422.3 | Forward | ATTCCATGGAGCCAGGCTTTC | 57 | 142 |
| Reverse | CATTTGGGTCAAACTCCACTGTG | ||||
| MMP-9 | NM_004994.2 | Forward | CATCCGGCACCTCTATGGTC | 57 | 637 |
| Reverse | CATCGTCCACCGGACTCAAA | ||||
| MMP-13 | NM_002427.3 | Forward | AAATTATGGAGGAGATGCCCATT | 57 | 125 |
| Reverse | TCCTTGGAGTGGTCAAGACCTAA | ||||
| TIMP-2 | NM_003255.4 | Forward | GTAGTGATCAGGGCCAAAGC | 57 | 160 |
| Reverse | GGGGGCCGTGATAAACT | ||||
| GAPDH | NM_001256799.2 | Forward | AGAAGGCTGGGGCTCATTTG | 52 | 271 |
| Reverse | AGGGGCCATCAGTCTTC | ||||