Figure 2.

The antibacterial activity of the cell-free supernatant obtained by sequential batch cultivation of the immobilized cells of Geobacillus sp. ZGt-1 against G. stearothermophilus strain 10 at 60 °C, at the end of (a) cycle # 1; (b) cycle # 5; (c) cycles # 11, 12, and 13; (d) cycles # 14, 15, 16, and 17; (e) cycles # 24 and 25. The yellow dot denotes the site where the supernatant was spotted; and (f) Summary of the antibacterial activity over the 25 cycles, the number of + symbols representing increasing degree of antibacterial activity.