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. 2016 Aug 19;17(8):1363. doi: 10.3390/ijms17081363

Figure 3.

Figure 3

Analysis of the antibacterial activity of the desalted protein fraction isolated from the culture supernatant produced by Geobacillus sp. ZGt-1 at 60 °C. The test organism was G. stearothermophilus strain 10. After SDS-PAGE separation of the protein in duplicates, the gel was divided into two, one part stained with Coomassie Brilliant Blue R 250 (a) and the other used for antibacterial assay (b). (a) Image of the SDS-PAGE separated protein fraction. Lane 1: Precision Plus Protein All Blue standards; Lane 2: Desalted protein fraction; (b) Antibacterial activity of the desalted protein extract after separating it on SDS-PAGE separated protein; the gel strip was placed in a Petri dish and covered with soft agar layer seeded with strain 10 and incubated at 60 °C. The white arrow is pointing to the inhibition zone ascribed to the antibacterial activity of the protein fraction. The inhibition zone corresponded to 15–20 kDa.