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. 2016 Aug 20;17(8):1370. doi: 10.3390/ijms17081370

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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MiR-132-3p inhibits the osteogenic differentiation of ligamentum flavum cells. (A) Endogenous miR-132-3p expression levels were measured via quantitative real-time polymerase chain reaction (qRT-PCR) at different time points during osteogenic differentiation of ligamentum flavum cells; * p < 0.05 compared with day 0; (B) miR-132-3p expression was assessed via qRT-PCR in ligamentum flavum cells transfected with miRNA mimics; * p < 0.05 compared with nonspecific microRNA (miR-NC) group; (C) miR-132-3p expression was assessed via qRT-PCR in ligamentum flavum cells transfected with miRNA inhibitors; * p < 0.05 compared with anti-miR-NC group; (D,E) Western blot analysis of osteogenic marker protein expression after miR-132-3p overexpression (D) and reduction (E) at day 14; (F,G) alkaline phosphatase (ALP) staining and Alizarin Red staining at day 14 showed ALP activity and calcification after miR-132-3p overexpression (F) and reduction (G). Scale bar represents 200 µm and is fitted for every figure.

MiR-132-3p inhibits the osteogenic differentiation of ligamentum flavum cells. (A) Endogenous miR-132-3p expression levels were measured via quantitative real-time polymerase chain reaction (qRT-PCR) at different time points during osteogenic differentiation of ligamentum flavum cells; * p < 0.05 compared with day 0; (B) miR-132-3p expression was assessed via qRT-PCR in ligamentum flavum cells transfected with miRNA mimics; * p < 0.05 compared with nonspecific microRNA (miR-NC) group; (C) miR-132-3p expression was assessed via qRT-PCR in ligamentum flavum cells transfected with miRNA inhibitors; * p < 0.05 compared with anti-miR-NC group; (D,E) Western blot analysis of osteogenic marker protein expression after miR-132-3p overexpression (D) and reduction (E) at day 14; (F,G) alkaline phosphatase (ALP) staining and Alizarin Red staining at day 14 showed ALP activity and calcification after miR-132-3p overexpression (F) and reduction (G). Scale bar represents 200 µm and is fitted for every figure.