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. 2016 Aug 20;17(8):1370. doi: 10.3390/ijms17081370

Figure 2.

Figure 2

MiR-132-3p directly targets FOXO1, GDF5 and SOX6. (A) The wild-type and mutated type miR-132-3p binding sites in the FOXO1, GDF5 and SOX6 3′-UTR. The sequences in red font showed the blinding sites; (B) The wild-type (WT) 3′-UTR or mutant (MUT) 3′-UTR reporter plasmids of the three genes were co-transfected into HEK293T cells with either miR-132-3p or miR-NC and fluorescence was quantified; * p < 0.05 compared with miR-NC group; (C,D) FOXO1, GDF5 and SOX6 protein expression levels were examined via Western blot following miR-132-3p mimics (C) and inhibitors (anti-miR-132-3p) (D) transfection in ligamentum flavum cells.