Skip to main content
. 2016 Aug 20;17(8):1370. doi: 10.3390/ijms17081370

Figure 3.

Figure 3

FOXO1, GDF5 and SOX6 knockdown inhibits osteogenic differentiation of ligamentum flavum cells. (A,B) FOXO1, GDF5 and SOX6 mRNA and protein expression levels examined via qRT-PCR and Western blot at different time points during osteogenic differentiation of ligamentum flavum cells; (C,D) FOXO1, GDF5 and SOX6 mRNA and protein expression level examined via qRT-PCR and Western blot following siRNAs transfection in ligamentum flavum cells; * p < 0.05 compared with non-targeting siRNA (si-NC) group; (E) Osteogenic marker protein expression examined via Western blot at day 14 after FOXO1, GDF5 or SOX6 knockdown; (F) ALP staining and Alizarin Red staining at day 14 showed inhibited ALP activity and calcification following FOXO1, GDF5 or SOX6 knockdown when compared with siNC group. Scale bar represents 200 µm and is fitted for every figure.