Table 4.

Primer sequences and protocol for amplifying cDNA products from mice

Gene	Sequence	Size (bp)	PCR
TRPV1*	F–CCT GCT CAA CAT GCT CAT TG	134	94°C for 3 min
	R–TCC TCA TGC ACT TCA GGA AA
TRPV3	F–CGT CAT GAT CCA GAA GGT CA	119	[94°C for 30 s, 63°C for 1 min, 72°C for 1 min (35 times)]
	R–TGT CCT TGG AGC ACT TCT CA
GAPDH	F–TGT GAA CGG ATT TGG CCG TA	250	72°C for 2 min 4°C, hold
	R–GGC CTC ACC CCA TTT GAT GT

^*Primer obtained from Jackson Laboratories.