Table 5.

Primer sequences and protocol for RT-PCR of single-cell rat nodose neurons

Gene	Sequence	Size, bp	PCR
TRPV1	F–GGC TGT CTT CAT CAT CCT GTT A	118	95°C for 3 min
	R–GTT CTT GCT CTC TTG TGC AAT C
TRPV3	F–GTG ACT CCA GAG GTC AGT AGA A	184	[95°C for 30 s, 60°C for 45 s, 72°C for 1 min (45 times)]
	R–TGG CCC TAA CAA CAG AAC ATA G
GAPDH	F–CGT GTT CCT ACC CCC AAT GT	153	72°C for 10 min, 4°C, hold
	R–ACA GGA GAC AAC CTG GTC CT