Table 3.

PCR primers, annealing temperatures, and product sizes for cDNA used in qRT-PCR

cDNA	Forward Primer (5′-3′)	Reverse Primer (5′-3′)	Anneal, °C	Product, bp
oCSH	ataaactccgaatccaaggtc	gttccttttgagtttgccag	58	177
oRPS15	atcattctgcccgagatggtg	tgctttacgggcttgtaggtg	58	124
oPol II	agtccaacatgctgacggacatga	agccaagtgccggtaattgacgta	60	332
oGAPDH	accactgtccactgccatcac	cctgcttcaccaccttcttga	60	268
oIGF1	tcgcatctctcttctatctggccctgt	acagtacatctccagcctcctcaga	62	238
oIGF2	gaccgcggcttctacttcag	aagaacttgcccacggggtat	62	202
oIGFBP1	tgatgaccgagtccagtgag	gtccagcgaagtctcacac	62	247
oIGFBP2	caatggcgaggagcactctg	tggggatgtgtagggaatag	55	330
oIGFBP3	ctcagagcacagacaccca	ggcatatttgagctccac	54	335

o, Ovine; PRR15, ribosomal protein S15; Pol II, polymerase II; IGF, insulin-like growth factor; IGFBP, IGF-binding protein.