In situ hybridization using a probe for the 16s rRNA of Wolbachia endobacteria. The 16s probe bound to Wolbachia in the lateral chords (A,D), early (A) and late (D) morulae, and microfilariae (F) of B. malayi. It also bound Wolbachia in the developing eggs of the cricket G. bimaculatus (B). No staining was detected in the late morulae (C) or microfilaria (E) of C. quiscali. Scale bars are all 25 µm. Species are designated within the figure as follows: Bm, B. malayi; Gb, G. bimaculatus; Cq, C. quiscali.