FIGURE 4.
Immunohistological detection of Wolbachia surface protein (WSP). WSP antibody labeled Wolbachia in the lateral chords (lc) and morula stage embryos in the uterus (ut) of B. malayi (A) and in developing eggs of G. bimaculatus (C). No staining was detected in morula stage (mo) embryos (D) or the ovaries (ov) (E) of C. quiscali. A positive control Hsp60 antibody labeled mitochondria in both B. malayi (B) and C. quiscali (F). Scale bars are all 25 µm. Species are designated within the figure as follows: Bm, B. malayi; Gb, G. bimaculatus; Cq, C. quiscali.