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pH effects on activity. (A) pH titration curve of recombinant IDOLDH prepared from crude culture media. All reactions were done with 13 mM racemic ipsdienol and 2 mM NADP+. Values are from a single replicate. (B) Effect of buffer composition on activity of IDOLDH prepared from infected cell lysates. Oxidation of (−)-ipsdienol by IDOLDH was measured in phosphate or Tris based buffers. Values are means of two replicates, ± maxima and minima.
