Fig. 5.

Stereo-selective ketone reduction. GC traces of products extracted from reactions of IDOLDH prepared from infected cell lysates incubated with NADPH and either ipsdienone (A) or ipsenone (B). Traces of authentic racemic ipsdienol and ipsenol are inset in grey. IDOLDH produced only the minus enantiomer of each alcohol. Similar results were seen with IDOLDH prepared from culture media (data not shown). Control Sf9 cell lysate reactions showed a 1–2% reduction of ketone to corresponding alcohol (not shown).