. 2016 Mar 6;160(3):141–151. doi: 10.1093/jb/mvw019

Table I.

IDOLDH substrate profile

Substrate^a	Concentration (μM)	Coenzyme	Specific activity (nmol·min⁻¹·mg⁻¹± SEE or std. err.^b)	N
(−)-Ipsdienol	17	NADP⁺	427 ± 25	62
(+)-Ipsdienol	17	NADP⁺	0.2 ± 0.2	1
Racemic ipsenol	17	NADP⁺	196 ± 8	3
Ipsdienone	17	NADPH	50 ± 4	16
Ipsenone	17	NADPH	92 ± 7	24
(−)-Menthone	17	NADPH	16 ± 0.1	2
Nerol	17	NADP⁺	2.6 ± 0.4	2
Geraniol	17	NADP⁺	1.5 ± 0.5	2
Citral	17	NADP⁺	2.5 ± 0.5	2
Myrcene	17	NADP⁺	0.22 ± 0.1	1
Myrcene	17	NADPH	4.4 ± 0.2	2
1,3 Butenediol	17	NADP⁺	3.0 ± 0.6	3
4-Methyl-2-pentanol	24	NADP⁺	1.0 ± 2.0	2
4-Methyl-2-pentanol	3,400	NADP⁺	19 ± 0.1	2
4-Methyl-2-pentanone	25	NADPH	5.9 ± 0.1	2
4-Methyl-2-pentanone	3,500	NADPH	70.9 ± 0.2	2
4-Methyl-3-penten-2-one	28	NADPH	3.9 ± 0.1	2
4-Methyl-3-penten-2-one	17,650	NADPH	24 ± 1	2
Acetone	90,000	NADPH	41 ± 1	4
10-Epi-ryanoid	21	NADP⁺	3.0 ± 1	1
8β-9α-ryanoid	22	NADP⁺	5.4 ± 0.6	1
3-Penten-2-ol	31	NADP⁺	13.6 ± 1.0	2
3-Penten-2-ol	5,110	NADP⁺	48.8 ± 1.0	2
β-Estradiol	20	NADP⁺	27.0 ± 0.8	1

Enzyme activity was determined by measuring changes in absorbance at 340 nm. The coenzyme concentration in all cases was 200 μM NADP⁺ or 167 μM NADPH. See Materials and Methods for standard assay conditions.

^aOther substrates tested but showing no activity included α-ketobutyrate, 1 - and 2-propanol and ethanol.

^bStandard error of the estimate (SEE) is a measure of the quality of the data with smaller values indicating a higher probability the model is correct. SEE is reported when N = 1, N = 2; Std. err. is reported when N ≥ 3.