Table III.

K_m and V_max values of putative substrates

Row	Substrate	[Constant Substrate]	Km ± SEE	Vmaxa	Normalized	Relative	Normalized
			(μM)	(nmol ·min−1·mg−1)	Vmaxb	Vmaxc	Vmax/Km (uM−1)
1	(−)-Ipsdienol	8.66 mM NADP	13 ± 5	639 ± 94	1.68	1.26	0.129
2	(−)-Ipsdienol	0.866 mM NADP	4 ± 2	523 ± 50	1.33	1.00	0.333
3	(−)-Ipsdienold	0.866 mM NADP	527 ± 44	2,590 ± 70	1.18	0.89	0.00224
4	(−)-Ipsdienole	0.866 mM NADP	22 ± 4	1,820 ± 90	1.07	0.80	0.0486
5	Racemic Ipsenol	0.866 mM NADP	9 ± 4	230 ± 45	0.58	0.44	0.0644
6	Ipsdienone	0.167 mM NADPH	26 ± 15	49 ± 19	0.21	0.16	0.00808
7	Ipsdienonee	0.167 mM NADPH	2 ± 0.6	380 ± 10	0.18	0.14	0.0900
8	Ipsenone	0.167 mM NADPH	3 ± 3	113 ± 28	0.4	0.30	0.133
9	(−)-Menthone	0.167 mM NADPH	20 ± 10	28 ± 11	0.05	0.04	0.00250
10	NADP	17.6 μM (−)-Ipsdienol	0.08 ± 0.04	517 ± 89	1.26	0.95	15.8

^aV_max is expressed as specific activity

^bCalculated by dividing the V_max of the experimental by the specific activity of the normalization assay (see Normalization assay).

^cCalculated by dividing the normalized V_max of the experimental by the normalized V_max of the preferred substrate, (−)-ipsdienol.

^dAssays done with 40PPIDOLDH (IDOLDH partially purified with 40% ammonium sulphate) within the first week of protein preparation.

^eAssays done with 40PPIDOLDH after the first week of protein preparation.