Table 2.
Proton Currents in Mammalian Phagocytes and Other Primary Tissue Cells
| Cell | Species | °C | pHo | pHi |
IH,max (pA) |
IH,max (pA/pF) |
Reference |
|---|---|---|---|---|---|---|---|
| Neutrophil | Human | 20.5 | 7.4 | 7.0 | 10 | - | (82) |
| Neutrophil | Human | 20 | 7.0 | 7.0 | 22a | - | (91) |
| Neutrophil | Human | RT | 7.4 | 7.3 | 107a | - | (211) |
| Neutrophil | Mouse | RT | 7.4 | 7.3 | 63a | - | (211) |
| Neutrophil | Mouse | 22.5 | 7.0 | 7.0 | 70 | - | (69) |
| Neutrophil | Mouse | 24 | 7.0 | 6.0 | - | 336 | (134) |
| Neutrophil | Mouse | RT | 7.5 | 6.5 | 58.6 | - | (135) |
| Neutrophil | Mouse | RT | 7.4 | 6.6 | - | 43 | (268) |
| Eosinophil | Human | 22.5 | 7.0 | 7.0 | 193 | - | (83) |
| Eosinophil | Human | 20 | 7.5 | 5.7 | - | 338 | (84) |
| Eosinophil | Human | 20.5 | 7.0 | 7.0 | 115a | - | (89) |
| Basophil | Human | 21 | 7.0 | 5.5 | 102 | - | (267) |
| Basophil | Human | 22.5 | 7.0 | 7.0 | 106a | - | (203) |
| Mast cell | Mouse | 32 | 7.3 | 7.3 | 12 | 2.4 | (184) |
| Macrophage | Mouse | 22 | 7.5 | 6.5 | - | 13.2 | (180) |
| Macrophage | Mouse | 22 | 7.5 | 6.8 | - | 11.1 | (85) |
| Macrophage | Rat | RT | 6.5 | 6.5 | 150 | - | (269) |
| Osteoclast | Rabbit | 22 | 7.5 | 6.0 | 267 | 6.7 | (185) |
| Osteoclast | Mouse | 22 | 7.3 | 7.4 | - | 3.4 | (266) |
| Monocyte | Human | 22 | 7.5 | 6.0 | 209 | 3.45 | (183) |
| Monocyte | Human | 21.5 | 7.0 | 7.0 | 175* | - | (92) |
| Microglia | Mouse | 21.5 | 7.4 | 5.8 | 275 | - | (270) |
| Microgliab | Mouse | 35 | 7.4 | 7.3 | 239 | 6.3 | (271) |
| Microgliab | Mouse | RT | 7.2 | 5.5 | 428 | 12.6 | (138) |
| Microglia | Mouse | 21.5 | 7.5 | 6.0 | 1488 | 52.2 | (272) |
| Microglia | Mouse | 21.5 | 7.5 | 6.0 | 1016 | - | (273) |
| Microglia | Rat | RT | 7.2 | 5.5 | 23 | 1.0 | (138) |
| Microglia | Rat | 23 | 7.3 | 6.8 | - | 5.4 | (274) |
| Microglia | Human | RT | 7.5 | 5.5 | 1200 | - | (138) |
| Dendritic cell | Mouse | RTc | 7.0 | 7.0 | - | 29.6 | (98) |
| T lymphocyte | Mouse | 24 | 8.0 | 6.0 | 294 | - | (137) |
| T lymphocyte | Human | 21.5 | 7.5 | 6.0 | 1.46 | 0.9 | (275) |
| B lymphocyte | Human | 21.5 | 7.5 | 6.0 | 170 | 94.7 | (275) |
| B lymphocyte CLL | Human | 21 | 7.0 | 7.0 | 135 | - | (224) |
| Fibroblast | Human | 24 | 7.4 | 6.2 | - | 1.1 | (276) |
| Alveolar epithelium | Rat | 20 | 7.0 | 5.5 | 334 | 27.3 | (163) |
| Alveolar epithelium | Rat | 20 | 7.0 | 6.5 | - | 17 | (164) |
| Sperm | Human | 24 | 7.4 | 5.5 | - | 104.3 | (277) |
| Sperm | Mouse | 24 | 7.4 | 5.5 | - | 3.6 | (277) |
| Chondrocyte | Cow | 21 | 7.0 | 6.0 | - | 7.2 | (278) |
| Myotube | Human | 21 | 7.33 | 7.0 | 19 | 0.1 | (279) |
Currents in cells stimulated with PMA (enhanced gating mode).
These microglia were in brain slices.
RT is room temperature.
For this table, IH,max is simply the largest current reported or depicted in each study. Some authors normalize the currents according to the size of the cell, whose membrane surface area is indicated by the capacity (in pF). Comparisons under different conditions are imprecise because proton currents are exquisitely sensitive to temperature (168, 280) and pH, and measurements were made at different voltages. When multiple pH were studied, the measurement nearest to physiological was selected. All cells are freshly isolated or in primary culture. When a temperature range is reported, the median value is given.